Main Idea Of The Road Not Taken - Free Essay Example

Sample details Pages: 2 Words: 571 Downloads: 3 Date added: 2019/07/31 Category Literature Essay Level High school Tags: The Road Not Taken Essay Did you like this example? The Road Not Taken, written by Robert Frost, uses an extended metaphor of a walking path to emphasize how a choice he makes at any moment could have a significant influence on his future. Frost begins the poem by talking about the diverging roads he stumbled upon in the woods. He realizes he is only one person and can only take one path, so he examines them both in an attempt to make the best decision. The decision was not easy for him as he states, long I stood before he made the choice (3). To the best of his ability he studies both paths, but he is not able to see much due the paths curving and being enveloped with trees. Frost implies that he would like to obtain more information about each path by saying, And looked down one as far as I could, but nature prevented him from doing so (4). Don’t waste time! Our writers will create an original "Main Idea Of The Road Not Taken" essay for you Create order In the second stanza, Frost examines both paths in depth; he notices that the second path is more attractive. He concludes that no one has taken it lately because it was grassy and wanted wear (8). He seems to be very indecisive though, declaring that the second path is just as pleasant as the first. The speaker seems to be searching for a logical reason to travel down one path over the other, but the reason is unobtainable. Frost continues to search for a logical reason to choose a certain path in stanza three. He continues to analyze the paths in hopes of finding something to make his decision simpler, but both paths are almost identical. He observes that neither path has been traveled lately because the leaves have not been walked on. It starts to become apparent to Frost that he is not going to find a clear reason to choose a certain path. The speaker knows he needs to make a decision, so he decides to take the second path. He tells himself that he will come back to the forest and take the first path another day. Frost uses an exclamation mark after line 13 to express his excitement of finally making a decision and being able to walk down both paths eventually. His excitement quickly weakens when reality sets in. He acknowledges that one road can lead to another and that he may end up very far from where he started. He doubts he should ever come back, as much as he wishes he could. The tone in the last stanza, stanza four, changes drastically. The speaker jumps forward in time to reflect on his decision. He says he will be telling this story somewhere ages and ages hence, which implies that the story is important to him (17). He then proceeds to repeat the first line of the poem, two roads diverged in a yellow wood, with a few modifications; he removes the word yellow and inserts the words and I (1). By doing this the speaker has emphasized that important part of the poem, the idea of choosing between two different paths. The speaker sums up his story in the last two lines of the poem. Frost states that he took the one less traveled by; even though he said the paths were almost equal, he believes that the one he chose was a little less worn (19). He believes that taking the path that was less worn than the other made all the difference in his life (20).

Essay about Mental Health Drugs - 1568 Words

As the second decade of the 21st century progresses, the population approaches seven billion. With so many people, how are people supposed to stand out in job applications, or catch the administrators’ eye as he or she reads applications to highly prestigious colleges and universities? More and more people are asking this question, and more and more people are finding help in a small pill. Originally diagnosed for Alzheimer’s disease and ADHD, these drugs are increasingly used off label in universities and workplaces. In society, people call this form of off label use of neuroenhancing drugs chiefly two different things: smart drugs informally, and nootropics formally. The word nootropic originated from a Romanian Dr. Corneliu E.†¦show more content†¦Companies manufacturing these drugs then work around this by lobbying their drugs as cures for Alzheimer, ADHD, ADD, dementia, dyslexia, and other cognitive defects. In the 1960s and ‘70s, the military ordered amphetamines and shipped them out to soldiers in Vietnam as a stimulant to keep them awake and aware for longer periods, while in the United States these amphetamines such as methamphetamine and cocaine became a recreational drug used in parties and a way to â€Å"be cool†. Compare this with today’s cognitive enhancing smart drug, which is still an amphetamine: the military is testing these smart drugs on soldiers to make them more effective with less sleep and prolonged battle and in the States, people use them off label to avoid jetlag or to write 9 page papers in 2 hours. In both these scenes, these drugs have received little coverage until they became popular and their effects became realized. â€Å"The effects of chronic, high doses of amphetamine are toxic; it can cause psychosis, depression and cognitive deficits, which are sometimes irreversible†, says Maia Szalavitz of Time Magazine (Popping). Although Piracetam and Modafinil are not amphet amines, drugs like Adderall and others used to treat attention-deficit hyperactivity disorder (ADHD) are amphetamines and are very popular in the cognitive enhancing world. That is why nootropics are dangerous; hardworking students andShow MoreRelatedMental Health, Gender And Drug Addiction1236 Words   |  5 PagesDiscussion Within the analysis of the dissertation, it has been recognised that there were three key themes associated with my findings: mental health, gender and drug addiction. We will dis-cuss the three documents chosen and highlight the themes which occurred. Though gender didn’t appear as much in two of the documents, it has relevance to the dissertation and was titled within the MoJ document, which recorded a high degree of statistics relating to gender. Following on from the three themedRead MoreEssay Drug Abuse and Mental Health 1194 Words   |  5 Pagesaspect of care for the person with a mental disorder. When drugs enter the brain, they can interrupt the work and actually change how the brain performs its jobs; these changes are what lead to compulsive drug use. Drug abuse plays a major role when concerning mental health. It is very difficult for these individuals to engage in treatment. Diagnosis for a treatment is difficult because it takes time to disengage the interacti ng effects of substance abuse and the mental illness. It may also be difficultRead MoreThe Use Of Recreational Drugs And Its Effects On Mental Health2286 Words   |  10 Pagesuse of recreational drugs has been common among humans ever since their psychoactive effects and ability to alter consciousness was discovered. A downside to this is that according to research, substance use has been linked to the development and expression of latent mental illness (a mental disorder that a person may carry the genes for but not express). My main research focus is the question of how drug use, especially hallucinogenic drugs, mediates and effects mental health, and whether it contributesRead MorePhysical And Mental Health Implications Of Drug Abuse Rehabilitation Programs1018 Words   |  5 PagesAs drugs have been abused for hundreds of years all over the world, their effects have been felt for just as long. Since drugs have been used, there were always those who abused them, which led to full-blown addiction and the bevy of side effects that come with it. As the physical and mental health implications of addiction became clearer, rehabilitation efforts began to appear. As a result, the history of rehabilitation in the United States dates back hundreds of years. Today, thousands of drug abuseRead MoreMental Health, Tobacco Regulation, And Medical Marijuana1063 Words   |  5 Pagesintroduced at the state level were associated with mental health, tobacco regulation, and medical marijuana. The first bill is HB 1072, which seeks to grant prescriptive authority to psychologists who meet specific education, training, and registration requirements. Supporters include the Hawaii State Center for Nursing, Hawai’i Medical Association (HMSA), and the Coalition for a Drug-Free Hawai’i (CDFH). Due to the widespread shortage of mental health professionals in Hawai’i, these supporters believeRead MoreSummarise and Discuss the Presentations of Mental Health in the Two Newspaper Articles Given in Appendix 1.1704 Words   |  7 PagesSummarise and discuss the presentations of mental health in the two newspaper articles given in Appendix 1. In this essay, I will summarise how both newspaper articles in Appendix 1 present mental health. I will also compare and contrast the articles with each other, as well as compare them to what I know about mental health and the history behind it including psychopharmaceuticals and psychotherapies. The first article, titled ‘six in ten of us have faced mental issues such as stress or depression’Read MoreThe Stigma Of Mental Health968 Words   |  4 PagesINTRODUCTION The stigma in mental health system is deep rooted and its origin goes back by centuries. The stigma is powerful that it has been codified in federal since last 50 years. But the realization that the stigma attached adversely affects mental health care system is still lacking. The discrimination against mental illness has invaded the systemic structure causing more worries to sufferers of mental illness and their families. While every individual and system is well aware of mental illness and theRead MoreMental Health And Rural And Remote Practice1661 Words   |  7 Pagescontrasting two chosen fields of practice being mental health and rural and remote practice. This paper will discuss a brief historical outline in regards to both fields, together with a statistical overview of certain challenges and concerns that occur within the field of mental health and rural and remote practice. An emphasis will placed on the challenges faced by the client, while comparing and contrasting issu es such as social disadvantage, infrastructure, drugs and alcohol misuse. Likewise, challengesRead MoreA Child s Mind Stands At Risk Of Stressors That Induce Development Of Behavioral Changes1107 Words   |  5 Pagescharacteristics of drug addiction include compulsive, at times uncontrollable drug craving, seeking, and use despite devastating consequences behaviors that stem from drug-induced changes in brain structure and function. Due to the commonality in underlying brain deficits, genetic vulnerabilities, and/or early exposure to stress or trauma, it is often impossible to differentiate the changes in areas of brain function when comparing symptoms of drug abuse and mental illness (National Institute on Drug Abuse,Read MoreMental Illness And Its Effects On A Person s Life1628 Words   |  7 PagesAll mental illnesses fit into five main categories; anxiety disorders, mood disorders, schizophrenia/psychotic disorders, eating disorders, and dementias. These illnesses have cripp ling effects on a person’s life. They change someone’s physical health, how they interact with the world, and how they perceive and address situations. An occurrence that would have no effect on someone with no mental issue, distorts the view of someone with a mental illness, into either a negative or positive issue, and

Briefing Report Business Model

Question: Discuss about theBriefing Report for Business Model. Answer: Business Model The website is built on WordPress platform and its URL is www.emarket101.cloudaccess.host.The website is an online book platform where buyers can search, view, and purchase books. It primarily focuses on digital books hence does not require shipping fees and is not restricted by location. The books are acquired and uploaded by the website administrator. In other words, eMarket101 can be described as one seller online platform. The business model primarily focuses on sale of digital books to the customers. Users who visit the site can search the books they want. The website offers a search functionality where users can search books related to their keyword. Also, users can view books by categories as presented on the homepage. Once a user identifies the book he wants, he adds it to the cart and checks it out. Buyers receive the books they have bought in their email. eMarket101 has a limitless stock of books since it deals with digital products. In addition, users are offered discounts based on the number of books they purchase. Website Structure and Purpose of Each Element The website consists of several pages with different purposes. The homepage includes a slider, informational widgets, and social media links. The image slider in the homepage includes images that offer the users a clear preview of what the website is all about. The homepage includes informational widgets which presents the websites proposition value and inform the users what they can get in the website. The social media links indicates the various social media accounts of the website. The about us page reveals the purpose of the company and its mission. It informs what the users should expect from the company. Company policies page include privacy and confidentiality, security, and returns and refunds policies which informs the user what they should follow when purchasing the products and how to respond in case an issue is encountered, and the responsibility of the company. The contact us page includes a contact form through which users who have questions or complaints can use to communicate with the company. In the products page, various products that the company offers are listed and includes an add to cart option where users can select a product an add it to cart. In the checkout page, users have an option to update cart and a form to fill in order to complete their purchases. The transactions page displays the purchases that a customer has made. The account page displays a customers shipping/account details and shipping address details which can be edited usin g the contact form displayed. WordPress Components and Features used in Development of Website Prototype During the development of the ecommerce website prototype for the company, various WordPress plugins were used. The plugins include MetaSlider, Contact Form Maker, Page Builder by SiteOrigin, SiteOrigin Widgets Bundle, and WP eCommerce. MetaSlider was used to create the Image slider on the homepage which includes various images that showcase the purpose of the website. Contact Form Maker supports the contact form in the contact us page including providing captcha code and verifying users input. The website prototype uses various tools offered by Page Builder which customize the structure and outlook of various pages. SiteOrigin Widgets Bundle includes a wide variety of widgets that are used to customize the homepage. The homepage includes several widgets which include text, picture, and social media widgets. WP eCommerce supports the overall functionality of the prototype as an online ecommerce platform. WP eCommerce includes functionality to manage products, create discounts, manage sales, payment integration, and tracking inventory. Components and Features to Include in the Final Website In the final ecommerce website, I plan to add an array of features to support its functionality and ensure a smooth user experience. I will include deals and discounts feature to attract people to the website. The website will feature shopping discounts and deals for various products offered. The website will offer discounted periods when the customers can enjoy the best deals. In addition, the website will have a loyalty feature which will recognize and reward customers loyalty based on their shopping frequency. The homepage will modified to display latest news and popular products. News including sales period or upcoming events will be displayed to inform the buyers about products offered. Buyers can find shopping deals and best product prices from the latest news featured on the homepage. Furthermore, customers will look through popular items on sale featured to find items that trending. The website will include a live chatting feature where buyers can interact with the customer service or sales team. When the customers encounter a problem, they can talk with the teams to clarify on issues encountered. The chatting feature will add value to the customer service and support the functions of the store support team. Instead of using laborious registration forms which are likely to make customers abandon items during checkout, the website will give buyers an option to checkout as guests. Offering them guest checkouts will enable them to make purchases quickly (Kalseek, 2012). The final website will include a feature that allow customers to give testimonials which will be displayed on the homepage. The testimonials will not only include text but also a photograph or image representing the buyer and will be shown randomly on the homepage and other webpages. A blog will be another feature on the final website that the company will use to publish content about its products. The blog is a vital SEO strategy for the company and an essential tool to reach out to the buyers to inform them about products offered and increase brand awareness (Fishkin, 2013). As part of the ecommerce website, the blog will be adapted to the website design to ensure a consistent look and appearance. The website will include an advanced on-site search engine. The ecommerce website will use a reliable search feature that allow filtering and has more search options for a more refined search of the inventory of products. As such, customers will effectively search products and received results that match what they look for. Also, the website will include a predictive feature which will analyze the buying patterns of the buyer and display products that are related to their previous purchases. Changes Implemented in the prototype that deviates from proposal and presentation in Assignment 1 The website prototype deviates slightly from what was presented in the proposal. The prototype includes an option for cart, checking out cart items, payment integration, and a transactions module which are not in the proposal. Despite the changes, the prototype covers all aspects and features that were proposed. References Fishkin, R., Hgenhaven, T. (2013).Inbound marketing and SEO: Insights from the Moz Blog. John Wiley Sons. Kalsbeek, M. (2012). Interface and interaction design patterns for e-commerce checkouts.

ELISA Test

Question: Discuss about theELISA Test. Answer: Overview This review aims to describe a rapid diagnostic assay using a Protein Technology. The assay that has been selected is ELISA Test. ELISA refers to Enzyme Linked Immunsorbent Assay. It is a well-known method and is most widely used rapid diagnostic assay. It is typically performed for detecting the presence and/or quantity of a target protein present in an experimental sample. The method of detecting the target protein is made possible through antibodies that make this technique as an immunoassay1. This test has been utilized as a diagnostic tool in the plant pathology and medicine. It is also utilized as a quality-control test in different industries. There are different types of ELISA Test namely, Indirect ELISA, Direct ELISA, Sandwich ELISA and Competitive and Inhibition ELISA. It is an important tool to determine the concentrations of serum antibody and it is a simple, versatile, sensitive and quantitative test. Method ELISA is concerned with the detection of an analyte, which is a specific substance and its presence is analyzed either qualitatively or quantitatively in a liquid medium through a method, which continues to utilize the liquid reagents throughout the analysis. The method of ELISA involves the separation of a number of components of an analytical reaction blend through the adsorption of certain constituents on a solid phase that has been physically immobilized. The addition of a liquid sample is carried on a stationary solid phase having exceptional binding properties and it is followed by numerous liquid reagents, and their addition, incubation and washing is carried out and is followed by a few visual changes such as development of color by the product of the enzymatic reaction 2. (Source: Takwoingi, Leeflang and Deeks. 2013) The sensitivity of process of detection is dependent on the signal amplification in the course of the analytical reactions. The generation of signal takes place by the enzymes that are linked foe detecting the reagents in fixed quantities. This is the reason, why this assay is given the enzyme linked. This test comprises of the components such as coated plates, substrate, conjugate, sample diluent, wash concentrate, controls and stop solution. The equipments of this test comprise of Dilutors, pipettes, ELISA plate readers and washer systems. Some of the other equipments include Incubator and Humidity chamber 3. The first step of this method involves the coating of antibody, in which the immobilization of a specific antibody is carried out on the plates that are meant for binding of a specific protein and is kept for overnight incubation. The blocking of plates is carried out with an unrelated protein such as albumin 7. The second step involves capturing the protein. This step is initiated with the addition of samples as well as standard solutions and their capturing takes place by the bound antibodies. The third step involves the detection of antibody in which the addition of specific biotinylated detection antibody to the wells is carried out for enabling the detection of the protein that has been captured8. In the fourth step, the addition of Streptavidin conjugated with horseradish peroidase (HRP) or alkaline phosphatise carried out and this leads to the binding with the biotinylated antibody. In the next step, addition of colorimetric substrate to the wells is performed and it results i n the formation of a coloured solution when it is catalyzed through the enzyme. In the final step, the analysis is performed by measuring the absorbance in a ELISA reader and the determination of the amount of protein is also carried out 4. (Source: Takwoingi, Leeflang and Deeks. 2013) Usefulness and Cost of the Technology ELISA is useful for detecting the presence of an antigen on antibody within a sample. It plays a significant role for determining the concentrations of serum antibody in a virus test. This test has an important application in the food industry for the detection of allergens in the food products. It is also applied in the situations of outbreak of disease for keeping a track on the spreading of diseases such as STDs, cholera and HIV6. It is also used to detect the antibodies in the blood sample for previous exposure to diseases like trichinosis and Lyme disease. The successful detection of antigens such as pregnancy hormones can be easily carried out by this test. It has proved to be an accurate quantitative method that can be used for the serodiagnosis of toxoplasmosis. The tests of ELISA are usually inexpensive and the companies that manufacture the test kits keep a low-cost for the customers 5. It is performed for detecting the presence and/or quantity of a target protein present i n an experimental sample. The method of detecting the target protein is made possible through antibodies that make this technique as an immunoassay. It has been utilized as a diagnostic tool in the plant pathology and medicine. It is also utilized as a quality-control test in different industries (Source: Takwoingi, Leeflang and Deeks. 2013) Comparison with DNA or other Technologies The ELISA test is different with DNA and other technologies as ELISA is a test which is used for detecting the presence of proteins in a sample, instead of DNA i.e. this test cannot be used for the detection of DNA 4. On the other hand, the tests like Southern blotting; Recombinant DNA technology is utilized for detecting DNA in animal or plant sample. DNA technology involves a set of diverse techniques, which allows the researchers foe examining, changing and creating new genetic substance. It ranges from the identification of criminals, creation of new biological products and involves the work of individuals from diverse disciplines. It also helps to understand the mechanisms of life, to solve mysteries and crimes and create solutions for nutrition along with healthy. Therefore, it differs largely in comparison to the ELISA test 6. To sum up, the ELISA test is a simple and a rapid diagnostic assay and offers a variety of advantages over the other techniques that are used in proteomics or protein technology in an efficient manner. Therefore, it plays a significant role and has varied applications that makes it a choice by the scientists and researchers. References Heick J, Fischer M, Kerbach S, Tamm U, Popping B. Application of a liquid chromatography tandem mass spectrometry method for the simultaneous detection of seven allergenic foods in flour and bread and comparison of the method with commercially available ELISA test kits. Journal of AOAC International. 2010 Dec;94(4):1060-8. Takwoingi Y, Leeflang MM, Deeks JJ. Empirical evidence of the importance of comparative studies of diagnostic test accuracy. Annals of internal medicine. 2013 Apr 2;158(7):544-54. Gan SD, Patel KR. Enzyme immunoassay and enzyme-linked immunosorbent assay. Journal of Investigative Dermatology. 2013 Sep 30;133(9):1-3. Singhal S, Mittal V, Khare V, Singh YI. Comparative analysis of enzyme-linked immunosorbent assay and direct microscopy for the diagnosis of Giardia intestinalis in fecal samples. Indian Journal of Pathology and Microbiology. 2015 Jan 1;58(1):69. Burrows AS. Matrix Effects on the Detection of Milk and Peanut Residues by Enzyme-Linked Immunosorbent Assays (ELISA). Heginbotham A, Millay V, Quick M. The Use of Immunofluorescence Microscopy and Enzyme-Linked Immunosorbent Assayas Complementary Techniques for Protein Identification in Artists' Materials. Journal of the American Institute for Conservation. 2013 Jul 18. Vashist SK, Schneider EM, Luong JH. Rapid sandwich ELISA-based in vitro diagnostic procedure for the highly-sensitive detection of human fetuin A. Biosensors and Bioelectronics. 2015 May 15;67:73-8. Zhou F, Wang M, Yuan L, Cheng Z, Wu Z, Chen H. Sensitive sandwich ELISA based on a gold nanoparticle layer for cancer detection. Analyst. 2012;137(8):1779-84.

ELISA Test

Question: Discuss about theELISA Test. Answer: Overview This review aims to describe a rapid diagnostic assay using a Protein Technology. The assay that has been selected is ELISA Test. ELISA refers to Enzyme Linked Immunsorbent Assay. It is a well-known method and is most widely used rapid diagnostic assay. It is typically performed for detecting the presence and/or quantity of a target protein present in an experimental sample. The method of detecting the target protein is made possible through antibodies that make this technique as an immunoassay1. This test has been utilized as a diagnostic tool in the plant pathology and medicine. It is also utilized as a quality-control test in different industries. There are different types of ELISA Test namely, Indirect ELISA, Direct ELISA, Sandwich ELISA and Competitive and Inhibition ELISA. It is an important tool to determine the concentrations of serum antibody and it is a simple, versatile, sensitive and quantitative test. Method ELISA is concerned with the detection of an analyte, which is a specific substance and its presence is analyzed either qualitatively or quantitatively in a liquid medium through a method, which continues to utilize the liquid reagents throughout the analysis. The method of ELISA involves the separation of a number of components of an analytical reaction blend through the adsorption of certain constituents on a solid phase that has been physically immobilized. The addition of a liquid sample is carried on a stationary solid phase having exceptional binding properties and it is followed by numerous liquid reagents, and their addition, incubation and washing is carried out and is followed by a few visual changes such as development of color by the product of the enzymatic reaction 2. (Source: Takwoingi, Leeflang and Deeks. 2013) The sensitivity of process of detection is dependent on the signal amplification in the course of the analytical reactions. The generation of signal takes place by the enzymes that are linked foe detecting the reagents in fixed quantities. This is the reason, why this assay is given the enzyme linked. This test comprises of the components such as coated plates, substrate, conjugate, sample diluent, wash concentrate, controls and stop solution. The equipments of this test comprise of Dilutors, pipettes, ELISA plate readers and washer systems. Some of the other equipments include Incubator and Humidity chamber 3. The first step of this method involves the coating of antibody, in which the immobilization of a specific antibody is carried out on the plates that are meant for binding of a specific protein and is kept for overnight incubation. The blocking of plates is carried out with an unrelated protein such as albumin 7. The second step involves capturing the protein. This step is initiated with the addition of samples as well as standard solutions and their capturing takes place by the bound antibodies. The third step involves the detection of antibody in which the addition of specific biotinylated detection antibody to the wells is carried out for enabling the detection of the protein that has been captured8. In the fourth step, the addition of Streptavidin conjugated with horseradish peroidase (HRP) or alkaline phosphatise carried out and this leads to the binding with the biotinylated antibody. In the next step, addition of colorimetric substrate to the wells is performed and it results i n the formation of a coloured solution when it is catalyzed through the enzyme. In the final step, the analysis is performed by measuring the absorbance in a ELISA reader and the determination of the amount of protein is also carried out 4. (Source: Takwoingi, Leeflang and Deeks. 2013) Usefulness and Cost of the Technology ELISA is useful for detecting the presence of an antigen on antibody within a sample. It plays a significant role for determining the concentrations of serum antibody in a virus test. This test has an important application in the food industry for the detection of allergens in the food products. It is also applied in the situations of outbreak of disease for keeping a track on the spreading of diseases such as STDs, cholera and HIV6. It is also used to detect the antibodies in the blood sample for previous exposure to diseases like trichinosis and Lyme disease. The successful detection of antigens such as pregnancy hormones can be easily carried out by this test. It has proved to be an accurate quantitative method that can be used for the serodiagnosis of toxoplasmosis. The tests of ELISA are usually inexpensive and the companies that manufacture the test kits keep a low-cost for the customers 5. It is performed for detecting the presence and/or quantity of a target protein present i n an experimental sample. The method of detecting the target protein is made possible through antibodies that make this technique as an immunoassay. It has been utilized as a diagnostic tool in the plant pathology and medicine. It is also utilized as a quality-control test in different industries (Source: Takwoingi, Leeflang and Deeks. 2013) Comparison with DNA or other Technologies The ELISA test is different with DNA and other technologies as ELISA is a test which is used for detecting the presence of proteins in a sample, instead of DNA i.e. this test cannot be used for the detection of DNA 4. On the other hand, the tests like Southern blotting; Recombinant DNA technology is utilized for detecting DNA in animal or plant sample. DNA technology involves a set of diverse techniques, which allows the researchers foe examining, changing and creating new genetic substance. It ranges from the identification of criminals, creation of new biological products and involves the work of individuals from diverse disciplines. It also helps to understand the mechanisms of life, to solve mysteries and crimes and create solutions for nutrition along with healthy. Therefore, it differs largely in comparison to the ELISA test 6. To sum up, the ELISA test is a simple and a rapid diagnostic assay and offers a variety of advantages over the other techniques that are used in proteomics or protein technology in an efficient manner. Therefore, it plays a significant role and has varied applications that makes it a choice by the scientists and researchers. References Heick J, Fischer M, Kerbach S, Tamm U, Popping B. Application of a liquid chromatography tandem mass spectrometry method for the simultaneous detection of seven allergenic foods in flour and bread and comparison of the method with commercially available ELISA test kits. Journal of AOAC International. 2010 Dec;94(4):1060-8. Takwoingi Y, Leeflang MM, Deeks JJ. Empirical evidence of the importance of comparative studies of diagnostic test accuracy. Annals of internal medicine. 2013 Apr 2;158(7):544-54. Gan SD, Patel KR. Enzyme immunoassay and enzyme-linked immunosorbent assay. Journal of Investigative Dermatology. 2013 Sep 30;133(9):1-3. Singhal S, Mittal V, Khare V, Singh YI. Comparative analysis of enzyme-linked immunosorbent assay and direct microscopy for the diagnosis of Giardia intestinalis in fecal samples. Indian Journal of Pathology and Microbiology. 2015 Jan 1;58(1):69. Burrows AS. Matrix Effects on the Detection of Milk and Peanut Residues by Enzyme-Linked Immunosorbent Assays (ELISA). Heginbotham A, Millay V, Quick M. The Use of Immunofluorescence Microscopy and Enzyme-Linked Immunosorbent Assayas Complementary Techniques for Protein Identification in Artists' Materials. Journal of the American Institute for Conservation. 2013 Jul 18. Vashist SK, Schneider EM, Luong JH. Rapid sandwich ELISA-based in vitro diagnostic procedure for the highly-sensitive detection of human fetuin A. Biosensors and Bioelectronics. 2015 May 15;67:73-8. Zhou F, Wang M, Yuan L, Cheng Z, Wu Z, Chen H. Sensitive sandwich ELISA based on a gold nanoparticle layer for cancer detection. Analyst. 2012;137(8):1779-84.